connective tissue reaction to white and gray mta mixed with distilled water or chlorhexidine in rats

introduction: the purpose of this study was to compare the histocompatibility of white (wmta) and gray (gmta) mineral trioxide aggregate mixed with 0.12% chlorhexidine (chx) and distilled water (dw) in subcutaneous connective tissues of rats. materials and methods: the freshly mixed wmta and gmta with chx or dw were inserted in polyethylene tubes and implanted into dorsal subcutaneous connective tissue of 50 wistar albino rats; tissue biopsies were collected and were then examined histologically 7, 15, 30, 60 and 90 days after the implantation procedure. the histology results were scored from 1-4; score 4 was considered as the worst finding. data were analyzed using one-way anova tests. results: all experimented materials were tolerated well by the connective tissues after 90-day evaluation, except for the wmta/chx group that had significantly more mean inflammatory scores (p<0.001). there was a statistically significant difference in the mean inflammation grades between experimental groups in each interval (p<0.001). after 90 days, gmta/chx group had the lowest inflammatory score. conclusion: although adding chx to wmta produces significantly higher inflammatory response, it seems a suitable substitute for dw in combination with gmta. further research is necessary to recommend this mixture for clinical use.